LIN-28 plays an important role as a translational enhancer, leading specific mRNAs to polysomes and therefore increasing the competence of protein synthesis. LIN-28 is a marker of undifferentiated human embryonic stem cells and it enhances the efficiency of the formation of induced pluripotent stem (iPS) cells from human fibroblasts. LIN-28 binds to the let-7 pre-miRNA and blocks production of the mature let-7 microRNA in mouse embryonic stem cells. Overexpression of LIN-28 is associated with human germ-cell tumors. Human recombinant LIN-28 produced in E. coli is a single, non-glycosylated polypeptide chain of 191 amino acids (42-209) and a molecular mass of 21.1 kDa. LIN-28 is expressed with a 23 amino acid His-tag fused at the N-Terminus and purified by proprietary chromatographic techniques.
Human LIN28A expressed in E.coli
CAT# CSC-CTK0604-10 (10 μg); CAT# CSC-CTK0604-50 (50 μg)
Greater than 90% as determined by SDS-PAGE analysis.
Less than 1 EU/μg.
Sterile filtered, colorless solution containing 20 mM Tris-HCl (pH 8.0), 10% glycerol and 0.1 M NaCl.
Please centrifuge product briefly before opening vial. The dissolved protein can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
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