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ActoFactor™ Recombinant Human KIT ligand (E. coli)

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Cat.No.
CSC-CTK0290
Description
Recombinant Human Stem Cell Factor (rHu SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. rHu SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures. Creative Bioarray's rHu SCF is an 18.4 kDa protein containing 164 amino acid residues.
Species
Human
Product Overview
Human KITLG expressed in E.coli
Molecular Mass
18.4 kDa
Size
CAT# CSC-CTK0290-10 (10 μg); CAT# CSC-CTK0290-50 (50 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.
Activity
The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is < 2 ng/ml, corresponding to a specific activity of 5 x 10^5 units/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized from a sterile-filtered protein solution in 10 mM acetic acid.
Reconstitution
Please centrifuge product briefly before opening the vial. The lyophilized rHu SCF should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be further diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu SCF should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, we recommend the addition of a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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