IL-6 mediates its biological effects through the type I IL-6 receptor system that consists of two chains, IL-6R alpha and gp130. The IL-6R alpha chain is the binding component specific to IL-6; while the gp130 only transmits signals of IL-6 when bound to IL-6R alpha. The gp130 also can transmit signals from LIF, OSM, CNTF, IL-11 and CT-1 in conjunction with other receptor subunits. The low-affinity binding site for IL-6 is composed of IL-6R alpha alone. IL-6R alpha is expressed in a wide range of cells including T cells, fibroblasts and macrophages. Soluble IL-6R alpha which consists of only the extracellular domain of the IL-6R alpha chain, acts as an agonist of IL-6 activity at low concentrations. Recombinant human sIL-6R produced in E. coli is a single, non-glycosylated polypeptide chain containing 338 amino acids (20-357) corresponding to the mature IL-6R protein with a molecular weight of 42.25 kDa and an aminoterminal hexahistidine tag.
Human IL6R expressed in E.coli
CAT# CSC-CTK0232-20 (20 μg); CAT# CSC-CTK0232-100 (100 μg)
Greater than 90% as determined by SDS-PAGE and HPLC analysis.
Less than 1 EU/μg.
Sterile-filtered, clear solution (0.18 mg/ml). The His-tagged protein is supplied in 20 mM Tris (pH 8.0), 0.8 M urea and 50% glycerol.
Please centrifuge product briefly before opening vial. The protein solution can be further diluted into water or other aqueous buffers and stored at -20°C for future use.
Storage & Stability
Store at 4°C if the entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid repeated freeze-thaw cycles.
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