Human Interleukin-4 (rHu IL-4) is a 15 kDa protein containing 130 amino acid residues. rHu IL-4 is a potent lymphoid cell growth factor, which stimulates the growth and survivability of certain B-cells and T-Cells.
Human IL4 expressed in E.coli
CAT# CSC-CTK0223-10 (10 μg); CAT# CSC-CTK0223-50 (50 μg)
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
The specific biological activity was determined after lyophilisation in direct comparison with the WHO standard (WHO international standard 88/656, NIBSC) by a standard cell proliferation assay using a human factor-dependent cell line TF-1. The ED50 for this effect is <0.2 ng/ml, corresponding to a specific activity of 5.0 x 10^6 units/mg.
Lyophilized from sterile-filtered protein solution (1 mg/ml) in water with no additives.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration of 0.1-1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-4 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze/thaw cycles.
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