Interleukin-4 (IL-4) is a pleiotrophic cytokine produced by activated T cells, mast cells and basophils. IL-4 stimulates the activation and differentiation of B cells as well as other hematopoietic and non-hematopoietic cells. Recombinant human IL-4 is a 14 kDa protein containing 130 amino acid residues.
Human IL4 expressed in E.coli
A DNA sequence encoding mature human IL-4 was expressed in E. coli.
CAT# CSC-CTK0090-10 (10 μg); CAT# CSC-CTK0090-50 (50 μg)
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
The biological activity of human IL-4 was measured in a cell proliferation assay using the factor-dependent human cell line, TF-1. The ED50 for this effect is typically 0.05 - 0.2 ng/mL.
Endotoxin level is less than 0.1 ng per μg of the cytokine, as determined by the LAL method.
Lyophilized from a sterile-filtered solution in PBS containing 50 μg of bovine serum albumin per 1 μg of cytokine.
It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 5 μg /mL of the cytokine.
Storage & Stability
The lyophilized powder is stable for greater than six months at -20°C to -70°C. Reconstituted IL-4 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.
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