IRF-4 is a transcriptional activator, belonging to the IRF family. It binds to the interferon- stimulated response element (ISRE) of the MHC Class I promoter (but is not induced by Interferons). IRF-4 binds the immunoglobulin lambda light chain enhancer, together with Pu.1. It most likely plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. IRF-4 / MUM-1 is expressed in the nuclei and cytoplasm of plasma cells and a small percentage of germinal center (GC) B cells mainly located in the "light zone". Its morphologic spectrum ranged from that of centrocyte to that of a plasmablast/plasma cell, and it displayed a phenotype (MUM-1+/Bcl-6/Ki67 ) different from that of most GC B cells (MUM-1 /Bcl-6+/Ki67+) and mantle B cells (MUM-1 /Bcl-6 /Ki67). Human recombinant IRF-4 (aa 144-451) expressed in E. coli is fused to a GST-tag and purified by proprietary chromatographic techniques.
Human IRF4 expressed in E.coli
CAT# CSC-CTK0186-2 2 μg; CAT# CSC-CTK0186-10 (10 μg)
Greater than 90% as determined by SDS-PAGE analysis.
Sterile-filtered, colorless protein solution in 50 mM Tris-Acetate (pH 7.5), 1 mM EDTA and 20% glycerol.
Please centrifuge product briefly before opening vial. The dissolved protein can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
Store frozen at -20°C. Avoid multiple freeze-thaw cycles.
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