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ActoFactor™ Recombinant Human Interferon beta-1a (CHO)

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Cat.No.
CSC-CTK0185
Description
Proteins of this family play an important role in inducing non-specific resistance against a broad range of viral infections. They also affect cell proliferation and modulate immune responses. Produced by peripheral blood leukocytes and lymphoblastoid cells, IFNalpha is an acid stable molecule that signals through IFN-alpha/betaR, which is also used by IFN beta. Both IFNs have similar anti-viral activity and regulate expression of MHC class I antigens. Recombinant human Interferon-beta 1a produced in CHO (Chinese Hamster Ovarian) cells is a 20.0 kDa protein containing 166 amino acid residues. Due to glycosylation, IFN-β has an approximate MW of 22.3 kDa based on SDS-PAGE gel and Mass Spectrometry.
Species
Human
Product Overview
Human IFNA1 expressed in CHO cells
Molecular Mass
20 kDa
Size
CAT# CSC-CTK0185-20 (20 μg); CAT# CSC-CTK0185-100 (100 μg)
Expression System
CHO cells
Purity
Greater than 95% as determined by SDS-PAGE, FPLC and HPLC analysis.
Activity
Interferon beta 1a is fully biologically active when compared to a standard. Assay #1: Measured by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 20-30 ng/ml. Assay #2: Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of Greater than 1 x 10^7 units/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile-filtered through a 0.2 micron filter and lyophilized from 10 mM sodium citrate, 100 mM sodium chloride and 10 mM sucrose.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted IFN-beta should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze / thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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