USA USA: 1-631-626-9181 Europe Europe: 44-207-097-1828  
Search

Tissue samples, tissue arrays, cells, microorganisms, probes and services for your research.

Web Search    Google
Featured Products
Ordering Information

Payment methods we support:
✭ Invoice / Purchase Order
✭ Credit card

ActoFactor™ Recombinant Human Interferon alpha-1b (E. coli)

  • Specification
  • Recommended Products
Cat.No.
CSC-CTK0177
Description
At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end. Recombinant human Interferon-alpha (rHu IFN-alpha) is a lymphoid factor, which possesses potent anti-viral activity. rHu IFN-alpha 1b is a non-glycosylated 19.4 kDa single-chain protein containing 166 amino acid residues.
Species
Human
Product Overview
Human IFNA1 expressed in E.coli
Molecular Mass
19.4 kDa
Size
CAT# CSC-CTK0177-100 (100 μg); CAT# CSC-CTK0177-500 (500 μg)
Expression System
E.coli
Purity
Greater than 96% as determined by SDS-PAGE, FPLC and HPLC analysis.
Activity
IFN-alpha 1b is fully biologically active when compared to a WHO standard. The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 1 x 10^8 IU per mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized from a sterile-filtered protein solution (50 μg/ml) in PBS (pH 7.4) containing 4% mannitol and 1% HSA.
Reconstitution
Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted IFN-alpha should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze / thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
Customer Support & Price Inquiry
Please input "bioarray" as verification code.
CONTACT US USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: 

Europe
Tel: 44-207-097-1828
Email: