Myostatin (GDF-8), a member of the TGF-beta superfamily, is a potent and specific negative regulator of skeletal muscle mass. In serum, myostatin circulates as part of a latent complex containing myostatin propeptide and/or follistatin-related gene. The myostatin propeptide is known to bind and inhibit myostatin in vitro. This interaction is relevant in vivo, with a majority (>70%) of myostatin in serum bound to its propeptide. The myostatin propeptide is a negative regulator of myostatin in vivo. 28 kDa protein containing 243 amino acid residues of the human Myostatin Propeptide and 5 additional amino acid residues.
Human GDF8 expressed in E.coli
CAT# CSC-CTK0542-25 (25 μg); CAT# CSC-CTK0542-125 (125 μg)
Greater than 95% as determined by HPLC and SDS-PAGE analysis.
Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1 mg/ml) solution containing 10 mM acetic acid.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, is best stored desiccated at -20°C. Reconstituted rHu GDF-8 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.