FGF-9 is a heparin binding growth factor that belongs to the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-9 targets glial cells, astrocytes cells and other cells that express the FGFR 1c, 2c, 3b, 3c, and 4. Recombinant human FGF-9 is a 23.4 kDa protein consisting of 207 amino acid residues.
Human FGF9 expressed in E.coli
CAT# CSC-CTK0147-20 (20 μg); CAT# CSC-CTK0147-100 (100 μg)
Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 0.5 ng/ml, corresponding to a specific activity of Greater than 2 x 10^6 units/mg.
Less than 1 EU/μg.
Sterile-filtered through a 0.2 micron filter. Lyophilized from a protein solution in 10 mM Tris (pH 8.0), 50 mM NaCl.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile 10 mM Tris (pH 8.0) to a concentration of 0.1-1.0 mg mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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