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ActoFactor™ Recombinant Human Fibroblast growth factor 9 (E.coli)

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Cat.No.
CSC-CTK0147
Description
FGF-9 is a heparin binding growth factor that belongs to the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-9 targets glial cells, astrocytes cells and other cells that express the FGFR 1c, 2c, 3b, 3c, and 4. Recombinant human FGF-9 is a 23.4 kDa protein consisting of 207 amino acid residues.
Species
Human
Product Overview
Human FGF9 expressed in E.coli
Molecular Mass
23.4 kDa
Size
CAT# CSC-CTK0147-20 (20 μg); CAT# CSC-CTK0147-100 (100 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.
Activity
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 0.5 ng/ml, corresponding to a specific activity of Greater than 2 x 10^6 units/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile-filtered through a 0.2 micron filter. Lyophilized from a protein solution in 10 mM Tris (pH 8.0), 50 mM NaCl.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile 10 mM Tris (pH 8.0) to a concentration of 0.1-1.0 mg mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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