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ActoFactor™ Recombinant Human Fibroblast growth factor 19

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Cat.No.
CSC-CTK0170
Description
The FGF family plays central roles during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF- 19, a member of the FGF family, is a high affinity heparin dependent ligand for FGFR4. FGF-19 is expressed during the brain development and embryogenesis. Recombinant human FGF-19 is a 21.8 kDa protein containing 195 amino acid residues.
Species
Human
Product Overview
Human FGF19 expressed in E.coli
Molecular Mass
21.8 kDa
Size
CAT# CSC-CTK0170-25 (25 μg); CAT# CSC-CTK0170-125 (125 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE analysis.
Activity
The ED50 as determined by a cell proliferation assay using Balb/c 3T3 cells is 100-150 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized from a sterile-filtered protein solution in 5 mM Tris (pH 7.6) + 75 mM NaCl.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized FGF-19 should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into water or other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu FGF-19 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
Customer Support & Price Inquiry
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