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ActoFactor™ Recombinant Human Connective tissue growth factor (HEK)

  • Specification
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Cat.No.
CSC-CTK0592
Description
CTGF is a member of the CCN family of secreted cysteine rich regulatory proteins and is the major mitogenic and chemoattractant protein produced by umbilical vein and vascular endothelial cells. CTGF stimulates the proliferation and differentiation of chondrocytes, induces angiogenesis, promotes cell adhesion of fibroblasts, endothelial, and epithelial cells, and binds to IGF, TGF beta1, and BMP-4. Cell migration and adhesion are signaled through binding to specific cell surface integrins and to heparin sulfate proteoglycans CTGF (98 a.a.), a lower molecular weight isoform containing the C-terminal portion of the full length CTGF protein, exerts full heparin binding, cell adhesion, and mitogenic CTGF activity. Recombinant human CTGF produced in HEK293 cells, is a 36.67 kDa protein containing a total of 334 amino acid residues and 11 additional amino acid residues (Flag-tag).
Species
Human
Product Overview
Human CTGF expressed in HEK cells
Molecular Mass
N/A
Size
CAT# CSC-CTK0592-10 (10 μg); CAT# CSC-CTK0592-50 (50 μg)
Expression System
HEK cells
Purity
Greater than 90% as determined by SDS-PAGE analysis.
Activity
N/A
Endotoxin Level
N/A
Formulation
Filtered and lyophilized from a protein solution (0 .5 mg/ml) in 20 mM Tris buffer and 50 mM NaCl (pH 7.5).
Reconstitution
Please centrifuge product briefly before opening vial. The CTGF can be solubilized with sterile pyrogen-free water at a concentration of 0.5 mg/ml. Let pellet dissolve completely. Protein is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
Customer Support & Price Inquiry
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