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ActoFactor™ Recombinant Human Chemokine (C-X-C motif) ligand 12 (22-89)

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Cat.No.
CSC-CTK0016
Description
Stromal cell-derived factor-1 (SDF-1), also known as Pre-B cell growth stimulating factor (PBSF), is a chemokine secreted by bone marrow and other stromal cells. SDF-1 is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. SDF-1 was shown to be a ligand for fusin/LESTR, a CXCR4 type receptor that functions as a coreceptor for HIV-1 strains. Recently, SDF-1 was shown to induce lymphocyte homing, mature blood cell functional activation and stem cell growth inhibition. SDF-1 is also a chemoattractant for CD34+ hemopoietic progenitors. Human SDF-1a is synthesized as a precursor of 89 amino acid residues processed to the mature form by cleavage of the N-terminus.
Species
Human
Product Overview
Human CXCL12 (22-89) expressed in E.coli
Sequence
A DNA sequence encoding the mature human SDF-1a protein (aa residues 22-89) was expressed in E. coli.
Size
CAT# CSC-CTK0016-10 (10 μg); CAT# CSC-CTK0016-50 (50 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
Activity was determined by the ability to chemoattract 5 – 10 day cultured human T lymphocytes and the ED50 using this assay was typically 3 – 9 ng/ml.
Endotoxin Level
Endotoxin level is less than 0.1 ng per mg of the cytokine, as determined by the LAL method.
Formulation
Lyophilized from a sterile-filtered solution in 30% acetonitrile, 0.1% TFA containing 50 mg of bovine serum albumin per 1 mg of cytokine.
Reconstitution
It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 mg/ml of the cytokine.
Storage & Stability
The lyophilized sample is stable for greater than six months at -20°C to -70°C. Reconstituted SDF-1 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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