MIP-1b is a member of the b (CC) family of chemokines. It is produced by fibroblasts, monocytes, T and B lymphocytes, neutrophils, smooth muscle cells, mast cells tumor cell lines. MIP-1b is a chemoattractant for monocytes and T cells. In addition, MIP-1b potentiates early hematopoietic precursors growth. The mature protein contains 69 amino acid residue and migrates with an apparent Mr of 7.8 kDa. MIP-1b binds to CCR1, CCR5 and US28.
Human CCL4 expressed in E.coli
A DNA sequence (APMGSDPPTACCFSYTARKLPRNFVVDYYETSSLCSQPAVVFQTKRSKQVCADPSESWVQEYVYDLELN) encoding the mature human MIP-1b protein (Act-2 variant) was expressed in E. coli.
CAT# CSC-CTK0048-10 (10 μg); CAT# CSC-CTK0048-50 (50 μg)
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity was determined by ability to chemoattract cultured human monocytes or mouse BaF/3 cells transfected with hCCR5 and the ED50 for these effects are typically 10 - 30 ng/mL for monocytes and 3 -10 ng/mL for BaF/3 transfected hCCR5 cells.
Endotoxin level less than 0.1ng per 1 μg of the cytokine as determined by the LAL method.
Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% TFA, containing 50 μg of bovine serum albumin per 1 μg of cytokine.
It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 μg/mL of the cytokine.
Storage & Stability
Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted MIP-1b/CCL4 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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