NNT-1/BCSF-3 is a neurotrophic factor with B-cell stimulating capabilities. Expressed in lymph nodes and spleen, NNT-1/BCSF-3 binds and activates glycoprotein 130 (gp130) and leukemia inhibitory factor receptor member beta (LIFR-beta) and induces tyrosine phosphorylation of these receptors. In vitro, it supports the survival of chick embryo motor and sympathetic neurons. In mice, NNT-1/BCSF-3 induces serum amyloid A, causes body weight loss and B cell hyperplasia associated with increased in serum IgG and IgM. Recombinant human NNT-1/BCSF-3 is a 22.4 kDa protein containing 199 amino acid residues.
Human CLCF1 expressed in E.coli
CAT# CSC-CTK0486-10 (10 μg); CAT# CSC-CTK0486-50 (50 μg)
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
NNT-1/BCSF-3 weakly supports chick E8 DRG neurite outgrowth at a concentration of 1.0 ng/ml.
Less than 1 EU/μg.
Lyophilized from a sterile-filtered solution with no additives.
Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted NNT-1 should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.
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