TGF-β family members are key modulators of cell proliferation, differentiation, matrix synthesis, and apoptosis. As implied by their name, BMPs initiate, promote, and regulate the development, growth and remodeling of bone and cartilage. In addition to this role, BMPs are also involved in prenatal development and postnatal growth, remodeling and maintenance of a variety of other tissues and organs. Increasing evidence indicates that BMP-Smad signaling has a tumor suppressing activity and that BMPs can inhibit tumor growth. BMP-6 is abnormally expressed in breast cancer cell lines; however, its function in promoting breast cancer development is unknown. Recombinant human BMP-6 produced in HEK cells is a 26.2 kDa homodimeric glycoprotein consisting of two 117 amino acid subunits, which correspond to amino acid residues 397 to 513 of the full-length BMP-6 precursor.
Human BMP6 expressed in HEK cells
CAT# CSC-CTK0531-10 (10 μg); CAT# CSC-CTK0531-50 (50 μg)
Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.
Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.03-0.06 μg/ml.
Less than 1 EU/μg.
Sterile-filtered through a 0.2 micron filter and lyophilized from 10 mM Tris (pH 9.0).
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu BMP-6 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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