Featured Products
- HCC-78
- HDLM-2
- DOHH-2
- L-540
- MX-1
- NALM-6
- NB-4
- CAL-51
- SNB-19
- KYSE-520
- MKN-45
- BA/F3
- MS-5
- HCEC-B4G12
- NK-92
- PA-TU-8988S
- MONO-MAC-1
- PA-TU-8902
- Human Microglia
- Human Hepatic Stellate Cells
- Human Skeletal Muscle Cells (DMD)
- Human Schwann Cells
- Human Oral Keratinocytes (HOK)
- Human Cardiomyocytes
- Human Small Intestinal Epithelial Cells
- Human Colonic Epithelial Cells
- Human Intestinal Fibroblasts
- Primary Human Large Intestine Microvascular Endothelial Cells
- Human Small Intestinal Microvascular Endothelial Cells
- Human Retinal Pigment Epithelial Cells
- Human Hepatocytes
- Cynomolgus Monkey Lung Microvascular Endothelial Cells
- Cynomolgus Monkey Vein Endothelial Cells
- C57BL/6 Mouse Primary Mammary Epithelial Cells
- C57BL/6 Mouse Vein Endothelial Cells
- Rat Primary Kidney Epithelial Cells
- Rat Gingival Epithelial Cells
- Rabbit Lung Endothelial Cells
Our Promise to You
Guaranteed product quality, expert customer support
ActoFactor™ Recombinant Human Bone morphogenetic protein 4 (E. coli)
- Specification
- Recommended Products
Cat.No.
CSC-CTK0529
Description
Human Bone Morphogenetic Protein-2 (BMP-2) is a disulfide-bonded homo-dimeric protein with an apparent molecular weight of 26 kDa. BMP-4 regulates similarly to its nearest homologue BMP-2, diverse fundamental processes during embryonic development. BMP-4 and other BMP proteins are members of the transforming growth factor-beta (TGF-beta) superfamily of proteins and have great potential for medical therapeutic applications, in particular because they allow or at least accelerate the ossification of extensive bone lesions. Recombinant human BMP-4 produced in E. coli is a monomeric, non-glycosylated polypeptide chain of 116 amino acids and a molecular mass of 13,009 Dalton.
Species
Human
Product Overview
Human BMP4 expressed in E.coli
Molecular Mass
13 kDa
Size
CAT# CSC-CTK0529-10 (10 μg); CAT# CSC-CTK0529-50 (50 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE and HPLC analysis.
Activity
N/A
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a sterile-filtered, concentrated protein solution (1 mg/ml) in 30% acetonitrile and 0.1% TFA.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein can be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml (for highly concentrated dilutions we recommend reconstitution in sterile 100 mM acetic acid). This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu BMP-4 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles. Applications Can be used as a molecular standard (Western, ELISA) for studying secreted BMP-4, for preparing antibodies for the BMP-4 monomer and as molecule standard for the detection of secreted BMP-4 in reduced SDS-PAGE.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Customer Support & Price Inquiry
