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ActoFactor™ Recombinant Human Bone morphogenetic protein 4

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Cat.No.
CSC-CTK0039
Description
BMP-4 (Bone Morphogenetic Protein 4) is a member of the transforming growth factor β (TGF-β) superfamily. BMPs have been shown to be key regulators of embryogenesis and are known to play a role in the growth and differentiation of various cell types, including embryonic stem cells (ESCs), mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells. The mature recombinant human BMP-4 is a disulfide-linked homodimeric protein consisting of two 116 amino acid residue subunits, and is generated by the proteolytic removal of the signal peptide and propeptides. The monomer has a calculated molecular mass of approximately 13 kDa, but migrates as an approximately 22 kDa protein under reducing conditions in SDS-PAGE due to glycosylation.
Species
Human
Product Overview
Human BMP4 expressed in NS0 cells
Sequence
The DNA sequence encoding the human BMP-2 signal peptide and propeptide (amino acid residues 1 - 282 of human BMP-2) fused to the human BMP-4 mature chain (amino acid residues 293 - 408 of human BMP-4) (Wozney, J. et al., 1988, Science 242: 1528 - 1534) w
Size
CAT# CSC-CTK0039-10 (10 μg); CAT# CSC-CTK0039-50 (50 μg)
Expression System
NS0 cells
Purity
Greater than 95% as determined by SDS-PAGE and visualized by silver stain
Activity
The ED50 as measured by its ability to induce alkaline phosphatase production by mouse ATDC5 chondrogenic cells (Nakamura, K. et al., 1999, Exp. Cell Res. 250: 351) is typically 10 - 30 ng/mL.
Endotoxin Level
Endotoxin level is less than 1.0 EU per 1 μg of the cytokine, as determined by the LAL method.
Formulation
Lyophilized from a sterile-filtered solution in 30% acetonitrile, 0.1% TFA containing 50 μg of bovine serum albumin per 1 μg of cytokine.
Reconstitution
It is recommended that sterile 4 mM HCl containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 μg/mL.
Storage & Stability
The lyophilized powder is stable for greater than six months at -20°C to -70°C. Reconstituted BMP-4 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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