Recombinant human ProBMP-2 is the precursor of BMP-2 which has 3 intramolecular cystein bridges. It is a homodimeric, non-glycosylated polypeptide chain of 337 amino acids and a molecular mass of 85,726 Dalton.
Human BMP2 expressed in E.coli
CAT# CSC-CTK0522-10 (10 μg); CAT# CSC-CTK0522-50 (50 μg)
Greater than 95% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
Active protein. The ED50 was determined by the stimulating effect on the migration of C3T3 cells.
Less than 1 EU/μg.
Sterile-filtered, white, lyophilized (freeze-dried) powder. The protein was lyophilized from a concentrated (0.1 mg/ml), sterile solution containi ng 10 mM HCl (pH 2).
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu ProBMP-2 should be used immediately or stored long- term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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