There are several proteolytic fragments or specific domains of proteins that act as inhibitors of angiogenesis. These include fragments of plasminogen such as Angiostatin protein kringles 1-4 and kringles 1-5, Endostatin, Restin, PEX, the N-terminal fragment of prolactin, and the N- terminally truncated platelet factor. Angiostatin is a proteolytic protein fragment of plasminogen that is comprised of the first 4 kringle regions. Angiostatin is an angiogenesis inhibitor. Ang K1-4 prevents the growth of endothelial cells, and its systemic administration inhibits the growth of primary carcinomas in mice. Angiostatin Kringles 1-3 segment has a larger inhibitory activity than the Angiostatin Kringles 1-4 fragment. The protease-activated Angiostatin Kringles 1-5 is the most potent plasminogen fragment with over 50 times larger endothelial cell specific inhibitory activity. Angiostatin Kringles 1-5 systemic administration inhibits growth of fibrosarcoma and significantly reduces neovascularization. Angiostatin is a 38 kDa protein fragment of the plasminogen composed of the first 4 kringle domains of plasminogen. Angiostatin K1-4 is also named plasminogen kringles 1-4 and PK1-4. It is manufactured by the protelytic cleavage of plasminogen by a serine protease from several prostate carcinoma cell lines. The manufacturing of angiostatin by pancreatic cancer cells can be inhibited by TGF-beta 1 along with plasminogen activator inhibitor type-1 (PAI1).|Human Angiostatin K1-4, produced from human fluid, is a glycosylated polypeptide chain which migrates as a doublet at 50 kDa on SDS-PAGE analysis.
Plg from Human Fluid
CAT# CSC-CTK0125-10 (10 μg); CAT# CSC-CTK0125-50 (50 μg)
Greater than 98% as determined by SDS-PAGE.
Human Angiostatin Kringles 1-4 significantly inhibits basic-FGF induced endothelial cell proliferation and migration at concentrations ranging from 300 nM to 1.0 μM.
Lyophilized from a solution (1 mg/ml) containing 20 mM Hepes buffer (pH 8.2) and 20 mM NaCl.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into water or other aqueous buffers and stored at -20°C to -70°C for future use. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted Ang K1-4 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze/thaw cycles.
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