Adipocyte Medium (AdM) is developed as a maintenance medium used after differentiation of human preadipocytes to mature adipocytes in vitro. It is a sterile liquid medium, which contains all of the components necessary to support lipid accumulation in the maturing adipocytes. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.
Storage and Shipping
Store the basal medium at 4 °C, the AdGS, the FBS and the P/S solution at -20 °C. Protect from light. Gel ice.
AdM consists of 500 ml of basal medium, 12.5 ml of fetal bovine serum, 5ml of Adipocyte Growth Supplement and 5 ml of penicillin/streptomycin solution.
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