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CHO-DHFR-
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Cat.No.
CSC-C6243X
Description
Subclone of the parental line CHO which was derived from the ovary of an adult Chinese hamster in 1957; the cells are dehydrofolate reductase negative (dhfr-; Dukx cells); patented cell line
Species
Hamster
Source
ovary cells
Recommended Medium
90% SuperCult® AlphaMEM without Nucleosides without L-Glutamine + 10% h.i. FBS + 2 mM L-glutamine (in case other media, e.g. DMEM or IMDM, are used, hypoxanthine and thymidine should be added as supplements)
Culture Properties
monolayer
Morphology
epithelial-like adherent monolayer
Karyotype
Near diploid hamster karyotype with 20% polyploidy - 21(20-22)<2n> - distinctive CHO morphology
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, PCR assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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