Subclone of the parental line CHO which was derived from the ovary of an adult Chinese hamster in 1957; the cells are dehydrofolate reductase negative (dhfr-; Dukx cells); patented cell line
epithelial-like adherent monolayer
Near diploid hamster karyotype with 20% polyploidy - 21(20-22)<2n> - distinctive CHO morphology
Mycoplasma: negative in DAPI, microbiological culture, PCR assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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