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Immortalized Mouse Hepatic Stellate Cells-SV40
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Cat.No.
CSC-I9351L
Description
The Immortalized Mouse Hepatic Stellate Cells – SV40 were derived from C57/Bl6 WT mouse via enzymatic digestion and Percoll density gradient centrifugation. The primary cells were immortalized via transfection of the pCMV Simian Virus 40 Large T antigen and hygromycin B selection. The Immortalized Mouse Hepatic Stellate Cells – SV40 are useful in studies focusing on liver fibrosis since it is the major cell type involved in the formation of scar tissue. The cells are particularly applicable in studies associated with inflammatory responses, as NF-κB and inflammatory cytokines levels have been found to change in the Immortalized Mouse Hepatic Stellate Cells upon LPS stimulation.
Species
Mus musculus
Source
Liver
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Transfection of pCMV Simian Virus 40 Large T antigen
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) Genotype analysis;
2) NF-κB Activation Assay;
3) Cytokine ELISA Assays;
4) 3[H]-thymidine Cell Proliferation Assay.
2) NF-κB Activation Assay;
3) Cytokine ELISA Assays;
4) 3[H]-thymidine Cell Proliferation Assay.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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