ROS-50

Specification
Recommended Products

Cat.No.

CSC-C0563

Description

Established from the peripheral blood of a 69-year-old man with B cell acute lymphoblastic leukemia (B-ALL L3) at relapse; reported to carry the BCL2 rearrangement in the minor cluster region (mcr) in contrast to the major breakpoint region (MBR) of most cell lines

Species

Human

Source

B cell leukemia

Recommended Medium

80-85% RPMI-1640 + 15-20% h.i. FBS

Culture Properties

suspension

Morphology

round to polymorph cells growing singly or in clusters in suspension

Karyotype

Human hyperdiploid karyotype with 12% polyploidy - 45-48<2n>XY, +7, +8, der(7;17)(p10;q10), t(8;14;18)(q24;q32;q21), der(8)t(8;14;18) - carries rearrangements of BCL2, IGH@ and MYC - matches published karyotype

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -
Viruses: PCR: EBV -, HBV -, HCV -, HIV

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CM-1448Y

SuperCult® RPMI 1640 Medium

INQUIRY

CS-001Y

PremSera® FBS

INQUIRY

For research use only. Not for any other purpose.

Q & A
Customer Review

What is immunomagnetic cell separation?

Immunomagnetic separation (IMS) is a laboratory tool that can efficiently isolate cells out of body fluid or cultured cells. It can also be used as a method of quantifying the pathogenicity of food, blood or feces. DNA analysis have supported the combined use of both this technique and Polymerase Chain Reaction (PCR).

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Professional staff

I appreciate the technical staff at Creative Bioarray for helping me to optimize the culture conditions for tumor cells.

02 Dec 2022

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