Established in 1995 from a primary ductal infiltrating breast carcinoma of a 50-year-old Caucasian woman; cells are described to be estrogen- and progesterone-receptor negative, and to possess a point mutation in p53 and a five-fold amplification of MYC
Human highly-rearranged flat-moded hypertetraploid/hypopentaploid karyotype with 13% polyploidy - 92-107<4n>XX, -X, -X, +1, -3, -3, -3, +5, +5, +5, +6, +7, +7, -8, +10, -13, -13, -14, -15, -15, -16, -16, -17, -17, -18, -18, -18, -19, -19, -20, -20, -21, -
epithelial cells growing adherently in monolayers
Mycoplasma: negative in microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vime
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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