Established from the peripheral blood taken in 1991 at relapse of a high-grade B-NHL (leukemic transformation of mantle cell lymphoma, stage IV) diagnosed in a 58-year-old Caucasian woman with previous history of cervical carcinoma
spheroid cells growing in suspension, singly or in clumps
Human hypodiploid karyotype with 8% polyploidy - 44(39-44)<2n>XX, -12, -17, -18, +mar, add(1)(p22), del(3)(p14p23), i(8p), i(8q), add(9)(p22)x1-2, t(11;14)(q13;q32), add(13)(p12), add(18)(q21) - sideline with two copies of der(14) and der(9) - carries t(1
Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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