BONNA-12

Specification
Recommended Products

Cat.No.

CSC-C0301

Description

Established from the spleen of a 46-year-old man with hairy cell leukemia (HCL) at diagnosis in 1988; cells were described to be nagative for the HCL-associated mutation BRAF V600E

Species

Human

Source

hairy cell leukemia

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

lymphoblast-like cells growing as single cells and in clumps in suspension

Karyotype

Human hyperdiploid karyotype with 4% polyploidy - 47(45-47)<2n>X, -Y, +12, t(9;10)(p12/13;q12) - supernumerary ring or acf present in most cells

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD19 +, CD20 +, CD34 -, CD37 +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -
Viruses: ELISA: reverse transcriptase neg

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CM-1448Y

SuperCult® RPMI 1640 Medium

INQUIRY

CS-001Y

PremSera® FBS

INQUIRY

For research use only. Not for any other purpose.

Q & A
Customer Review

What is the alternative for FBS in cell culture?

Unfortunately, FBS can be replaced only by animal fluids to culture most of the cells in vitro. Therefore, we suggest that HPL-(HI-CF) can be a meaningful option to alternate FBS.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Reasonable prices

I consider Creative Bioarray products to be reasonably priced, which is very friendly to us.

28 Mar 2022

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