Porcine Pancreatic Fibroblasts

Specification
Recommended Products

Cat.No.

CSC-C4897L

Description

Porcine Ovarian Fibroblasts from Creative Bioarray are isolated from ovarian tissue of porcine. Porcine Ovarian Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution and incubated in Creative Bioarray Complete Growth Medium. The cells are shipped at passage 2. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen.

Species

Porcine

Source

Pancreas

Recommended Medium

SuperCult® Porcine Pancreatic Fibroblast Medium

Cell Type

Fibroblast

Disease

Normal

Quality Control

Porcine Primary Ovarian Fibroblasts are tested for negative expression of von Willebrand Factor Expression/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Porcine Primary Ovarian Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.

Storage and Shipping

Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.

Q & A
Customer Review

So how do you determine the logarithmic phase of a cell?

According to the above cell growth curve, in order to ensure viability, it is important to keep the cells in the logarithmic phase and pass them on, so it is important not to wait until the cells have grown to 100% fusion before digesting and passing them on. Generally, the cells can be passaged when they reach about 70% -80%.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Highly recommend

Creative Bioarray is one of the most reliable companies I have ever worked with. Their products and services are of the highest quality, and I highly recommend them.

12 Aug 2023

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