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SNB-19

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  • Cat.No.
  • CSC-C0391
  • Description
  • Established from the surgical resection of a left parieto-occipital glioblastoma from a 47-year-old man in 1980; cells were described to secrete plasminogen activator, to be clonogenic in soft agar and to be tumorigenic in nude mice; DNA fingerprinting showed unequivocally that SNB-19 is a subclone of the astrocytoma cell line U-251 MG)
  • Recommended Medium
  • 90% DMEM + 10% h.i. FBS
  • Organism
  • Human
  • Morphology
  • adherent fibroblastic cells growing as monolayer with contact inhibition, occasional giant cells
  • Culture Properties
  • monolayer
  • Karyotype
  • Human hypotriploid karyotype with 15% polyploidy - 63(58-63)<3n>XXY, +1, +7, -8, -10, -12, -13, -14, -15, -16, -18, -21, -22, +2mar, der(1)del(1)(q23)ins(1;4)(p32;q?23q27), del(1)(q13), del(4)(q23q27), del(4)(q28q35), add(8)(q24), add(11)(p15), der(19)add
  • Quality Control
  • Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, GFAP +, neurofilament -, vimentin +
    Viruses: ELISA:
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • astrocytoma

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USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com