Immortalized Cell Line Engineering Service
Immortalized cell lines and services are of ever-increasing importance in various aspects of biomedicine, toxicology and biotechnology. The cells can be used as model systems in phenotypic and drug screening, high content analysis, target identification, drug development, toxicology testing, gene editing studies, reporter assays. Besides, the immortalized cells have implications for the pharmaceutical, as well as cosmetic industries.
Creative Bioarray offers the establishment of novel, customer tailored cell lines. Customer tailoring of our immortalized cell lines means that cell lines can be established from tissues or donors that are specified by our customers, as well as that specific element (e.g. hormone responsive promoter constructs for analysis of biologicals, biosimilars or biobetters, or traceable fluorescent proteins, etc), are stable introduced or knock-out (using Crispr/Cas9). Thus, specific and easy read-outs are created for fast, efficient and reproducible cell-based assays using relevant, primary like cell lines. Projects can be started at any points as shown in the Figure 2.
By creating cell banks of in vitro immortalized, primary-like cells from different tissues and donors with diverse genetic backgrounds, you are enable to perform their research on more than one individual cell line. Thereby, Creative Bioarray products help to assess the efficacy or toxicity of drugs against the genetic background of general population. In addition, since the cells are primary-like but immortalized, sufficient cell material is available for establishing standardized, reproducible and relevant cell-based assays. And the immortalized cell lines will allow for sufficient and reproducible cell material for high-throughput and high content screening.
Apart from that, Creative Bioarray also provides Gene Editing in Immortalized Cell Lines. Creative Bioarray has >10 years in gene editing of human cells form various tissues and organs. And we have highest quality standards in generating cell lines (normal/immortalized cells, stem cells and differentiated cells), extended routine quality systems.
Creative Bioarray can run even complex projects, from tissue sourcing to cloning of genetic construct, characterization of cellular phenotype and bioassay development.
Reporter Cell Line
Figure 1. Establishment of Reporter Cell Lines ectopic expression of GFP in immortalized cells
CRISPR/Cas9 Engineering
Figure 2. Characterisation of RPTEC/TERT1 CD46 knock-out clone 1E3. a Immunofluorescence of E-cadherin and ZO1. b Gamma-glutamyltransferase activity. c FACS analysis of CD13 (Aminopeptidase N). D: Dome formation. (Wieser, Matthias, et al. "CD46 knock-out using CRISPR/Cas9 editing of hTERT immortalized human cells modulates complement activation." Plos one 14.4 (2019): e0214514.)
Full Service Workflow
Figure 3: Establishment of customer tailored cell lines
- Tissue sourcing
- Primary cell isolation
- Cell Immortalization
- CRISPR/Cas9 Based KO and KI or other genetic modification
(Reporter Cell Lines, CRISPR/Cas9 engineering)
- Cell screening
- Isolation of single clones using limiting dilution, FACS or MACS
- Cell line characterization
Highlights:
- Full service scope and start at any stage upon request
- Flexible options on immortalization methods: immortalized with lentiviruses (SV40T, hTERT) or Epigenetic Induction of Cells Growth Approach
- Ectopic expression of foreign genes/RNAs
- Highly qualified experts with decades of experience
- Deliver high-yield cell clones, detailed service report and provide technical support
- Cost-efficient & fast turnaround
Applications
- In vitro toxicity studies
- Phenotypic drug screening
- Target screening
- Testing of drug delivery and barrier functions
- Gene function studies using CRISPR/Cas9 including orthogonal drug screening
- Advanced batch release assays
Quotation and ordering
Call us today or email us for a quotation or if any questions! Our customer service representatives are available 24hr a day! We thank you for choosing Creative Bioarray at your preferred Immortalized Cell Line Engineering Service.
