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Porcine Aortic Endothelial Cells
- Specification
- Recommended Products
Cat.No.
CSC-C1755
Description
Porcine Aortic Endothelial Cells from Creative Bioarray are isolated from Porcine Aorta. Porcine Aortic Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and are delivered frozen. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic pre-coated with secondary antibody.
Species
Porcine
Source
Aorta
Recommended Medium
Porcine Endothelial Cell Medium
Cell Type
Endothelial
Disease
Normal
Quality Control
Porcine Aortic Endothelial Cells are tested for uptake of Dil-Ac-LDL (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. Porcine Aortic Endothelial Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.
Never can primary cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
During cell culture, it is found that the surface of adherent cells is stained with a lot of dead cells, and how to break it during the passaging process has been present?
A:
After washing it twice with PBS, add trypsin in, shake it, pour the trypsin out quickly, wash it again with PBS, and add trypsin for normal digestion.
Average Rating: 4.0 | 1 Scientist has reviewed this product
Good growth
The cells are in good growth condition during the experiment.
09 Dec 2022
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