Porcine Thymus Epithelial Cells

Specification
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Cat.No.

CSC-C9256J

Description

Porcine Thymus Epithelial Cells from Creative Bioarray are isolated from thymus tissue of porcine. Porcine Thymus Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’s Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.

Species

Porcine

Source

Thymus

Recommended Medium

Complete Epithelial Cell Medium

Cell Type

Epithelial

Disease

Normal

Storage and Shipping

We ship frozen cells on dry ice. Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. Never can primary cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.

Q & A
Customer Review

Possible reasons for the death or poor survival rate of purchased cells?

Incorrect use of medium or poor quality medium; incorrect use of serum or poor quality serum; incorrect thawing procedure; washing and centrifugation of frozen cells after thawing; suspension of cells mistaken for dead cells; incorrect use of incubation temperature; incubator gas concentration not meeting requirements; cells left at -80 °C for too long.

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Average Rating: 4.0 | 1 Scientist has reviewed this product

Patient help

The staff patiently helped me with various questions I encountered during the use of the cells, which enabled my experiments to be carried out and completed smoothly.

18 Feb 2023

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