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CAL-39

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-C0316
  • Description
  • Established from the primary lesion resected from a 76-year-old woman with bulky, moderately differentiated squamous cell carcinoma of the vulva in 1984; cells were reported to express an elevated number of high affinity EGF receptors, to carry gene amplification at 11q13 and to be tumorigenic in nude mice
  • Recommended Medium
  • 80% DMEM + 20% h.i.FBS + 0.5 nM hydrocortisone + 1 μg/100 ml EGF
  • Organism
  • Human
  • Morphology
  • adherent, epithelial-like cells growing as monolayer
  • Culture Properties
  • monolayer
  • Karyotype
  • Human flat-moded hypotriploid karyotype with 27% polyploidy - 59(54-60)<3n>XX, -X, +1, +1, +2, +3, +9, +12, +12, +16, +20, +22, +4mar, del(1)(q22), del(3)(p14), add(4)(p16), del(7)(q32), add(9)(p22), del(12)(q15), add(12)(q24), add(18)(q23) - additional 1
  • Source
  • vulva squamous cell carcinoma
  • Quality Control
  • Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
    Immunology: cytokeratin +, desmin -, endothel -, GFAP -, neurofilament -, vimentin -
    Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -,
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 0.75-1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com