Human Hypertension Artery Fibroblasts

Specification
Recommended Products

Cat.No.

CSC-C4996J

Description

Human hypertension artery fibroblasts are isolated from the carotid artery of human donor that has been diagnosed with hypertension. Human hypertension artery fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in complete growth medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml.

Species

Human

Source

Artery

Recommended Medium

SuperCult® Human Fibroblasts Growth Medium Kit

Cell Type

Fibroblast

Disease

Diseased

Quality Control

Human hypertension artery fibroblasts are negative for bacteria, yeast, fungi and mycoplasma.

Storage and Shipping

Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CM-1466J

SuperCult® Human Fibroblasts Growth Medium Kit

INQUIRY

For research use only. Not for any other purpose.

Q & A
Customer Review

What is the appropriate cell inoculation density?

It is sufficient to inoculate the cells at the density shown in the basic data of the cell line or at the ratio of the diluted plates. Too few cells or too much dilution is also an important reason for the failure of cell growth. According to our experience: for cells with a doubling time of 24 h, a passaging ratio of 1:6-1:12 is appropriate; for cells with a doubling time of 24-48 h, a passaging ratio of 1:3-1:8 is appropriate; for cells with a doubling time of more than 48 h, a passaging ratio of 1:2-1:4 is appropriate.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Wonderful

The cell product is of good quality with detailed step-by-step instructions.

24 Nov 2022

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