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Rat Bone Marrow Progenitor Endothelial Cells
- Specification
- Recommended Products
Cat.No.
CSC-C8693W
Description
Rat Bone Marrow Progenitor Cells are derived from the tibias and femurs of 1-week old Sprague Dewley Rat. Rat Bone Marrow Progenitor Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.
Species
Rat
Source
Bone Marrow
Cell Type
Progenitor; Endothelial
Disease
Normal
Quality Control
Rat Bone Marrow-Derived Endothelial Cells are tested for expression of markers using antibody, PECAM-1 Antibody (M-20, sc-1506, Santa Cruz) or ZO-1 Rabbit Polyclonal Antibody (Catalog No. 617300, Life Technologies) by immunofluorescence staining. The cells were tested for uptake of Dil-Ac-LDL, a functional marker for endothelial cells. Rat Bone Marrow Progenitor Cells are negative for bacteria, yeast, fungi and mycoplasma, and can be expanded for 3-6 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.
Never can primary cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
On the Creative Bioarray website I found that you offer bone marrow derived Endothelial Progenitor Cells, which would also be a sufficient cell source for what I am looking for. However, for me it would not be feasible to work with the cells from the sprague Dawley rats. Do you also offer bone marrow derived EPCs from either F344 (preferably), norway brown or Lewis Rats if requested?
A:
For the Rat Bone Marrow Progenitor Endothelial cells that you are looking for, F344, norway brown or Lewis Rats, we can do custom service.
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