Human Bladder Microvascular Endothelial Cells

Specification
Recommended Products

Cat.No.

CSC-C8606W

Description

Human Bladder Microvascular Endothelial Cells from Creative Bioarray are isolated from human bladder tissue. Human Bladder Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vails. Each vial contains at least 0.5×10^6 cells per ml. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.

Species

Human

Source

Bladder

Cell Type

Endothelial

Disease

Normal

Quality Control

Human Bladder Microvascular Endothelial Cells from Creative Bioarray display typical cobblestone with large dark nuclei appearance under light microscopy. Cells are tested for expression of endothelial cell marker using antibody CD31 (Catalog No. 550389, BD; CD31/PECAM-1 PE-conjugated Antibody, Catalog No. FAB3567P, R&D) or VE-Cadherin (FITC-VE-cadherin Catalog No. 560411, BD) by immunofluorescence staining or FACS. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased. Cells can be expanded for 3-5 passages under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.

Storage and Shipping

Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Never can cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.

Q & A
Customer Review

What passage number would they be?

You will receive Human Bladder Microvascular Endothelial Cells from passages 2 or 3, depending on cell purity.

How fast do the cells replicate?

Average doubling time ranges from 24-48 hours. However, keep in mind that the replication rate varies slightly from donor to donor.

Are antibiotics included in the medium?

Yes. Penicillin ,streptomycin and amphotericin B are included in the media.

How often should I change the media?

After thawing and plating the cryopreserved cells, the first medium change should be done after 24 hours or overnight, so that both residual DMSO and any dead cells are removed. Thereafter, the medium should be changed every 48 hours until the cells are ready to be passaged.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

It gives outstanding results

The cell did as expected in our study and it gave good results.

19 Feb 2023

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