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ActoFactor™ Recombinant Rat Interleukin 3

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0816
  • Description
  • Interleukin-3 (IL-3) is a cytokine produced by activated T-cells and mast cells that is active on a broad range of hematopoietic cells and in the nervous system and appears to be important in several chronic inflammatory diseases. It is a species-specific colony stimulating factor, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. Recombinant rat IL-3 produced in E. coli is a single, non-glycosylated polypeptide chain containing 140 amino acids and having a molecular mass of 15,783 Dalton. It has been purified by proprietary chromatographic techniques.
  • Species
  • Rat
  • Product Overview
  • Rat Il3 expressed in E.coli
  • Molecular Mass
  • 16 kDa
  • Size
  • CAT# CSC-CTK0816-20 (20 μg); CAT# CSC-CTK0816-100 (100 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.
  • Activity
  • Recombinant rat IL-3 is fully biologically active when compared to a standard. The ED50 as determined by the dose-dependant proliferation of murine NFS-60 cells is 3-10 ng/ml, corresponding to a specific activity of ≤3.3 x 10^5 IU/mg.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile-filtered white, lyophilized (freeze-drie d) powder. Lyophilized from a concentrated (1 mg/ml) protein solution with no additives.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rRa IL-3 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com