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ActoFactor™ Recombinant Mouse Sonic hedgehog-N

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0074
  • Description
  • Mouse Sonic Hedgehog (Shh-N) is an important signaling molecule expressed during embryonicdevelopment. It is involved in regulating the patterning of the developing central nervous system, somite and limb. mShh may also play a role in the development of whisker, hair, foregut, tooth and bone in the mouse embryo.
  • Species
  • Mouse
  • Product Overview
  • Mouse Shh-N expressed in E.coli
  • Sequence
  • A DNA sequence encoding amino acid residues Cys 25-Gly 198 of mouse Shh fused to a 6X histidine tag at the carboxy-terminus was expressed in E. coli.
  • Size
  • CAT# CSC-CTK0074-25 (25 μg); CAT# CSC-CTK0074-125 (125 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 97% as determined by SDS-PAGE and visualized by silver stain
  • Activity
  • Activity was measured by its ability to induce alkaline phosphatase production by C3H10T1/2 fibroblasts. The ED50 for this effect is 0.6 – 3 mg/mL.
  • Endotoxin Level
  • Endotoxin level is less than 0.1 ng per μg of the cytokine as determined by the LAL method.
  • Formulation
  • Lyophilized from a 0.2 μm filtered solution in PBS containing 5% Trehalose and 50mg of bovine serum albumin per 1mg of cytokine.
  • Reconstitution
  • It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 50 μg/mL of the cytokine.
  • Storage & Stability
  • Lyophilized samples are stable for greater than six months at -20°C to -70°C. Upon reconstitution, mouse Shh-N can be stored under sterile conditions at 2-4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
  • Official Symbol
  • Shh-N

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com