Online Inquiry & Support

USA: 1-631-626-9181   Fax: 1-631-614-7828
Europe: 44-207-097-1828
Email: info@creative-bioarray.com

ActoFactor™ Recombinant Mouse Interleukin 4

Bookmark and Share
  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0080
  • Description
  • Mouse Interleukin 4 (IL-4) is produced by activated Th2 cells, mast cells and basophils. IL-4 is a pleiotropic immune regulatory cytokine that promotes growth of human B cells; induces expression of germ line Cε transcripts, directs na?ve B cells to switch to the synthesis of IgE and IgG4, and induces expression of CD23 and ClassII-MHC. In monocytes, IL-4 down-regulates FcγR expression; inhibits synthesis of proinflammatory cytokines, including TNF-α, IL-1β, IL-6 and IL-8; induces monocyte/macrophage fusion and mannose receptor expression. IL-4 inhibits the synthesis of IL-12, nitric oxide synthase and cyclooxigenase-2. The mouse IL-4 cDNA encodes a 119 amino acid residue protein that has a molecular mass of 14 kDa. Mouse IL-4 is species specific. IL-4 binds to a heterodimeric receptor formed by the binding subunit IL-4Rα and the γ chain of IL-2R.
  • Species
  • Mouse
  • Product Overview
  • Mouse Il4 expressed in E.coli
  • Sequence
  • A DNA sequence encoding the mature mouse IL-4 protein minus the first two amino acid residues was expressed E. coli.
  • Size
  • CAT# CSC-CTK0080-10 (10 μg); CAT# CSC-CTK0080-50 (50 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 97% as determined by SDS-PAGE and visualized by silver stain
  • Activity
  • Activity was determined in a cell proliferation assay using a mouse factor-dependent cell line HT-2 and the ED50 using this assay was typically 1 – 2 ng/mL.
  • Endotoxin Level
  • Endotoxin level is less than 0.1 ng per μg of the cytokine, as determined by the LAL method.
  • Formulation
  • Lyophilized from a sterile-filtered solution in PBS containing 50 μg of bovine serum albumin per 1 μg of cytokine.
  • Reconstitution
  • It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 1 μg /mL of the cytokine.
  • Storage & Stability
  • The lyophilized sample is stable for greater than six months at -20°C to -70°C. Reconstituted mouse IL-4 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

Price Inquiry

Please input "bioarray" as verification code.

SEARCH

tissue samples, tissue arrays, cells, microorganisms, probes and services for your research.

  

SEARCH

Contact Us
    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
Ordering Information
    • Payment methods we support:
      ✭ Invoice / Purchase Order
      ✭ Credit card
Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com