Online Inquiry & Support

USA: 1-631-626-9181   Fax: 1-631-614-7828
Europe: 44-207-097-1828
Email: info@creative-bioarray.com

ActoFactor™ Recombinant Mouse Interleukin 13

Bookmark and Share
  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0002
  • Description
  • Mouse Interleukin-13 (IL-13) is produced primarily by activated Th2 cells. IL-13 is a pleiotropic immune regulatory cytokine that promotes growth of human B cells; induces expression of germ line Ce transcripts, directs na?ve B cells to switch to the synthesis of IgE and IgG4, and induces expression of CD23 and Class II-MHC. In monocytes, IL-13 down-regulates FcgR expression; inhibits synthesis of proinflammatory cytokines, including TNF-a, IL-1b, IL-6 and IL-8; induces monocyte/macrophage fusion and mannose receptor expression. IL-13 inhibits the synthesis of IL-12, nitric oxide synthase and cyclooxigenase-2. The mouse IL-13 cDNA encodes a 131 amino acid protein that shares 58% identity with human IL-13 and exhibits cross-species activity on human cells. Additionally, IL-13 shares 30% sequence homology to IL-4 and the two cytokines exert overlapping biological activities. IL-13 binds to a heterodimeric receptor formed by the IL-13Ra and the IL-4Ra. The mouse IL-13 cDNA encodes a 131 amino acid protein containing a proposed 20 amino acid signal peptide. The 106 amino acid residue recombinant protein has a predicted molecular mass of approximately 11.5 kDa. The purified protein migrates anomalously on SDS-PAGE as a 9 kDa protein.
  • Species
  • Mouse
  • Product Overview
  • Mouse Il13 expressed in E.coli
  • Sequence
  • A DNA sequence corresponding to amino acid residues Ser26 to Phe131 of the mouse IL-13 cDNA was expressed in E. coli.
  • Size
  • CAT# CSC-CTK0002-5 (5 μg); CAT# CSC-CTK0002-25 (25 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 97% as determined by SDS-PAGE and visualized by silver stain
  • Activity
  • Activity was determined in a cell proliferation assay using a human factor-dependent cell line, TF-1 and the ED50 for this effect is typically 3 - 6 ng/mL.
  • Endotoxin Level
  • Endotoxin level less than 0.1 ng per 1 mg of the cytokine as determined by the LAL method.
  • Formulation
  • Lyophilized from a sterile solution in PBS containing 50 mg of bovine serum albumin per 1 mg of cytokine.
  • Reconstitution
  • It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 5 mg/mL of the cytokine.
  • Storage & Stability
  • Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted mouse IL-13 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

Price Inquiry

Please input "bioarray" as verification code.

SEARCH

tissue samples, tissue arrays, cells, microorganisms, probes and services for your research.

  

SEARCH

Contact Us
    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
Ordering Information
    • Payment methods we support:
      ✭ Invoice / Purchase Order
      ✭ Credit card
Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com