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ActoFactor™ Recombinant Mouse Hepatocyte growth factor

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0729
  • Description
  • Hepatocyte Growth Factor (rMu HGF) is also known as Scatter Factor and Hepatopoeitin A. HGF is a potent mitogen for mature parenchymal hepatocyte cells and acts as a growth factor for a broad spectrum of tissues and cell types. Activities of HGF include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive. Murine HGF is expressed as a linear 728 amino acid polypeptide precursor glycoprotein. Proteolytic processing of this precursor generates the biologically active form of HGF, which consists of two polypeptide chains (alpha- chain and beta-chain) held by a single disulfide bond resulting in formation of a biologically active heterodimer. The alpha-chain consists of 463 amino acid residues and four kringle domains. The beta-chain consists of 233 amino acid residues.
  • Species
  • Mouse
  • Product Overview
  • Mouse Hgf expressed in Hi-5 Insect Cells
  • Molecular Mass
  • N/A
  • Size
  • CAT# CSC-CTK0729-20 (20 μg); CAT# CSC-CTK0729-100 (100 μg)
  • Expression System
  • Hi-5 Insect Cells
  • Purity
  • Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.
  • Activity
  • Recombinant human HGF is fully biologically active when compared to standards. Determined by the dose-dependent stimulation of the proliferation of mouse IMCD3 cells using a concentration range of 10-20 ng/ml.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile-filtered through a 0.2 micron filter and ly ophilized from 10 mM Tris (pH 7.2), 100 mM L- Arginine, 200 mM NaCl.
  • Reconstitution
  • Please centrifuge product briefly before opening the vial. The lyophilized recombinant human HGF should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at - 20°C for future use.
  • Storage & Stability
  • The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu HGF should be stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com