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ActoFactor™ Recombinant Human Vascular endothelial cell growth factor 121 (HEK)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0387
  • Description
  • Recombinant Human Vascular Endothelial Cell Growth Factor121 (rHu VEGF-121) is a 28.4 kDa homodimeric protein consisting of two non-glycosylated 121 amino acid polypeptide chains. VEGF-121 is a secreted homodimeric protein secreted by a variety of vascularized tissues. The reported activities of VEGF-121 include stimulation of endothelial cell growth, angiogenesis, and capillary permiability. VEGF-121 does not bind on heparin. Recombinant human VEGF-121 produced in HEK cells is a glycosylated 37 kDa homodimer and 50 kDa homotrimer. It has been purified by proprietary chromatographic techniques.
  • Species
  • Human
  • Product Overview
  • Human VEGFC expressed in HEK cells
  • Molecular Mass
  • N/A
  • Size
  • CAT# CSC-CTK0387-10 (10 μg); CAT# CSC-CTK0387-50 (50 μg)
  • Expression System
  • HEK cells
  • Purity
  • Greater than 95% as determined by SDS-PAGE analysis.
  • Activity
  • rHu VEGF-121 is fully biologically active when compared to standards. The specific activity was determined by the dose-dependent stimulation of the proliferation of HUVECs (Human Umbilical Vein Endothelial Cells) and is typically 2-8 ng/ml.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated protein solution (1 mg/ml) in 1xPBS.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu VEGF-121 should be used immediately or stored long- term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com