- TNFR2 is a member of the TNFR family of transmembrane proteins, and is expressed in immune cells and certain endothelial cells. It is a high affinity receptor for TNF-alpha but manifests a lower affinity to TNF-beta. Signaling through this receptor regulates various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. Soluble TNFR2 is capable of inhibiting TNF-alpha induced activities by acting as a decoy receptor. Recombinant human sTNFR2 is an 18.9 kDa protein (174 amino acid residues) comprising the cysteine rich ligand binding portion of the extracellular domain of the TNFR1 protein.
- Product Overview
- Human TNFRSF1B expressed in E.coli
- CAT# CSC-CTK0358-20 (20 μg); CAT# CSC-CTK0358-100 (100 μg)
- Greater than 97% as determined by SDS-PAGE and HPLC analysis.
- Determined by its inhibitory effect of the TNF-alpha mediated cytotoxicity in murine L-929 cells. The ED50 for this effect in the presence of 0.25 ng/ml of recombinant human TNF-alpha is 0.125 μg/ml.
- Endotoxin Level
- Less than 1 EU/μg.
- lyophilized from a sterile-filtered solution in 10 mM Sodium Phosphate, pH 8.0 + 50 mM NaCl.
- Please centrifuge product before opening vial. The lyophilized TNFR2 should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu sTNFR2 should be used immediately or stored long-term in undiluted working aliquots at -20°C.
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