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ActoFactor™ Recombinant Human Transforming growth factor, beta 3 (CHO)
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Cat.No.
CSC-CTK0331
Description
Transforming Growth Factor betas (TGF-betas) mediate many cell-cell interactions that occur during embryonic development. Three TGF-betas have been identified in mammals. TGF-beta 1, TGF-beta 2 and TGF-beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule. Human Recombinant TGF-b 3 produced in CHO cells is a disulfide-linked homodimeric, glycosylated, polypeptide chain containing 112 amino acids and having a molecular mass of 25 kDa.
Species
Human
Product Overview
Human TGFB3 expressed in CHO cells
Molecular Mass
25 kDa
Size
CAT# CSC-CTK0331-10 (10 μg); CAT# CSC-CTK0331-50 (50 μg)
Expression System
CHO cells
Purity
Greater than 97% as determined by SDS-PAGE analysis.
Activity
The biological activity was determined using a cell toxicity assay with HT-2 cells. The ED50 was found to be < 0.05 ng/ml.
Endotoxin Level
N/A
Formulation
Sterile-filtered, clear solution (10 μg/41 μl; 0.244 mg/ml) containing 20% ethanol and 0.1% acetic acid.
Reconstitution
Please centrifuge product briefly before opening vial. The dissolved protein can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
Store frozen at -20°C for longer periods of time. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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