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ActoFactor™ Recombinant Human Retinol binding protein 5, cellular
- Specification
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Cat.No.
CSC-CTK0513
Description
RBP-5 is a member of the Retinol-binding proteins family. Estimation of retinol-binding protein is used to determine visceral protein mass in nutritional studies related to health. RBP-5 has a higher expression in the adult kidney and liver and to a lesser extent in the adult and fetal spleen, adult lymph nodes and appendix, and fetal liver and kidney. RBP-5 expression is strongly decreased in hepatocellular carcinoma tissues. Human recombinant RBP-5 produced in E. coli is fused with a 20 amino acid His-tag at the N- terminus. It is a single, non-glycosylated polypeptide chain of 155 amino acids (1-135 a.a.) and a molecular mass of 18.1 kDa. It has been purified by proprietary chromatographic techniques.
Species
Human
Product Overview
Human RBP5 expressed in E.coli
Molecular Mass
18.1 kDa
Size
CAT# CSC-CTK0513-10 (10 μg); CAT# CSC-CTK0513-50 (50 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE analysis.
Activity
N/A
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile-filtered, colorless protein solution (0.5 m g/ml) containing 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 2 mM DTT, and 100 mM NaCl.
Reconstitution
Please centrifuge product briefly before opening vial. The dissolved protein can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
Store at 4°C if entire vial will be used within 1 week. Store frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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