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ActoFactor™ Recombinant Human Pro-Insulin Like Growth Factor 2 (HEK)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0209
  • Description
  • IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3 region and to the upstream INS gene at the 5 region. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. Recombinant human Pro-IGF-2 produced in HEK cells is a glycosylated monomer, having a total molecular weight of 25 kDa. It has been purified by proprietary chromatographic techniques.
  • Species
  • Human
  • Product Overview
  • Human IGF2 expressed in HEK cells
  • Molecular Mass
  • 25 kDa
  • Size
  • CAT# CSC-CTK0209-10 (10 μg); CAT# CSC-CTK0209-50 (50 μg)
  • Expression System
  • HEK cells
  • Purity
  • Greater than 95% as determined by SDS-PAGE analysis.
  • Activity
  • rHu Pro-IGF-2 is fully biologically active when compared to a standard. The specific activity was determined by the dose-dependent stimulation of the proliferation of MCF-7 cells (human breast cancer cell line) and is typically 2-8 ng/ml.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated protein solution (1 mg/ml) in 1xPBS.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze/thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com