- Liver X Receptors (LXRs) are nuclear receptors that regulate the metabolism of cholesterol and bile acids. There are two subtypes of LXRs, LXRα and LXRβ. The LXRs are ligand-dependent transcription factors that form permissive heterodimers with the retinoid X receptor (RXR). LXR- member of Nuclear Receptor Family is activated by certain oxysterol derivatives of cholesterol. They play an important role in cholesterol, lipid, and carbohydrate metabolism. LXRα is highly expressed in liver tissue. They respond to elevated cholesterol levels via transactivation of genes involved in sterol transport (ABCA1, ABCG1, ABCG5, and ABCG8), cholesterol efflux and high- density lipoprotein (HDL) metabolism, and sterol catabolism (CYP7A1). They also play a central role in regulating cellular lipid content through activation of SREBP-1c, which is the master regulator of de novo lipogenesis. LXRs were found to upregulate angiopoietin- like protein 3 (Angpf13), a member of the family of vascular endothelial growth factors that is also a key regulator of lipid metabolism. The livers X receptors are critical for the control of lipid homeostasis. LXRs serve as cholesterol sensors that regulate the expression of multiple genes involved in the efflux, transport, and excretion of cholesterol. Synthetic LXR agonists inhibit the development of atherosclerosis in murine models. These observations identify the LXR pathway as a potential target for therapeutic intervention in human cardiovascular disease. Recombinant human LXR produced by transient expression of LXR in non-transgenic plants contains a 6-His-tag at the N-terminal end and has a molecular weight of 31.9 kDa (201-461 amino acids). It is purified by sequential chromatography (FPLC) and does not contain any animal-derived components or impurities.
- Product Overview
- Human NR1H2 expressed in Nicotiana benthamiana
- CAT# CSC-CTK0654-10 (10 μg); CAT# CSC-CTK0654-50 (50 μg)
- Expression System
- Nicotiana benthamiana
- Greater than 97% as determined by SDS-PAGE analysis.
- Endotoxin Level
- Less than 0.04 EU/μg.
- Lyophilized from a 20 mM Tris-HCl buffer (pH 8.0), 0.1% SDS.
- Please centrifuge product before opening the vial. The lyophilized protein should be reconstituted in water to a concentration of 50 μg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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