Online Inquiry & Support

USA: 1-631-626-9181   Fax: 1-631-614-7828
Europe: 44-207-097-1828
Email: info@creative-bioarray.com

ActoFactor™ Recombinant Human Noggin

Bookmark and Share
  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0035
  • Description
  • Noggin is a member of the transforming growth factor-β family of ligands. Noggin antagonizes the activity of various bone morphogenetic proteins (BMP) by inhibiting access to their respective receptors. Since BMP cause human embryonic stem cells (hESC) to differentiate, noggin can be used to antagonize BMP signals and help maintain hESC in the undifferentiated state1. Recombinant human noggin is a single, non-glycosylated, polypeptide chain containing 206 amino acids and having a total MW of 23.1 kDa.
  • Species
  • Human
  • Product Overview
  • Human NOG expressed in E.coli
  • Sequence
  • Expressed in E. Coli
  • Size
  • CAT# CSC-CTK0035-20 (20 μg); CAT# CSC-CTK0035-100 (100 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 95% as determined by SDS-PAGE and visualized by silver stain
  • Activity
  • The ED50 of noggin, as measured by the ability to inhibit 5 ng/ml of BMP-4 induced alkaline phosphatase production by REFERENCES: 1. Xu RH, Peck RM, Li DS, Feng X, Ludwig T, Thomson JA. Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells. Nat Methods 2(3):185-90, 2005 Revised: June 2007 Recombinant Human NOGGIN Version 1.0.0
  • Endotoxin Level
  • Endotoxin level is less than 0.1 ng/μg (1.0 EU/μg) of recombinant human noggin.
  • Formulation
  • Lyophilized from a sterile filtered solution containing no additives.
  • Reconstitution
  • Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1 to 1.0 mg/ml. Note: For solubility reasons the protein should be kept at low pH. This solution can then be diluted into other aqueous buffers and stored at 2 - 8°C for 1 week or -20°C for future use.
  • Storage & Stability
  • Lyophilized samples are stable at roomtemperature (15 – 25°C) for 2 weeks, but it is recommended to store these desiccated at -20°C to -80°C. Reconstituted human noggin should be stored at 2 - 8°C for 1 week and for 6 months at -20°C to -80°C. Avoid repeated freezing and thawing.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

Price Inquiry

Please input "bioarray" as verification code.

SEARCH

tissue samples, tissue arrays, cells, microorganisms, probes and services for your research.

  

SEARCH

Contact Us
    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
Ordering Information
    • Payment methods we support:
      ✭ Invoice / Purchase Order
      ✭ Credit card
Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com