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ActoFactor™ Recombinant Human Mesencephalic astrocyte-derived neurotrophic factor

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0642
  • Description
  • MANF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non- neuronal tissues. It has been shown to promote survival, growth and function of dopamine specific neurons. MANF and its structural homolog CDNF, each contain an N-terminal saposin- like lipid binding domain, and a carboxyl-terminal domain, which is not homologous to previously characterized protein structures. MANF and CDNF can prevent 6-OHDA induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson disease. Recombinant human MANF is an 18.1 kDa protein consisting of 158 amino acids including 8 cysteine residues.
  • Species
  • Human
  • Product Overview
  • Human MANF expressed in E.coli
  • Molecular Mass
  • 18.1 kDa
  • Size
  • CAT# CSC-CTK0642-25 (25 μg); CAT# CSC-CTK0642-125 (125 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 98% as determined by SDS-PAGE and HPLC analysis.
  • Activity
  • Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 15-25 μg/ml.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile filtered through a 0.2 micron filter and ly ophilized from 10 mM sodium phosphate (pH 7.0).
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com