- Galectins are a family of animal lectins with an affinity for beta-galactosides. This family has at least 14 identified members. Galectins share similarities in the CRD (the carbohydrate recognition domain). Galectins are synthesized as cytosolic proteins. Though localized principally in the cytoplasm and lacking a classical signal peptide, galectins can also be stimulated to secretion by non-classical pathways or alternatively targeted to the nucleus. Galectins are involved in modulating cell-cell and cell-matrix interactions. Galectin-14 is a member of the galectin family of carbohydrate binding proteins. The Galectin-4 gene is predominantly intracellularly in the placenta and eosinophils and is released by eosinophils following allergen stimulation. Galectin-4 may be involved in the development of allergic inflammation. Human recombinant Galectin-14 produced in E. coli is fused with a 23 amino acid His-tag at the N-terminus. It is a single, non-glycosylated polypeptide chain of 162 amino acids (1-139 a.a.) and a molecular mass of 18.5 kDa. It has been purified by proprietary chromatographic techniques.
- Product Overview
- Human LGALS14 expressed in E.coli
- CAT# CSC-CTK0587-5 (5 μg); CAT# CSC-CTK0587-25 (25 μg)
- Greater than 90% as determined by SDS-PAGE analysis.
- Endotoxin Level
- Less than 1 EU/μg.
- Sterile filtered, colorless solution (0.5 mg/ml) co ntaining 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl and 1 mM DTT.
- Please centrifuge product briefly before opening vial. The dissolved protein can be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- Store at 4°C if entire vial will be used within a week. Store frozen at -20°C for longer periods of time. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
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